Development of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes

Publication Overview
TitleDevelopment of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes
AuthorsZhai C, Xu P, Zhang X, Guo Q, Zhang X, Xu Z, Shen X
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Year2015
CitationZhai C, Xu P, Zhang X, Guo Q, Zhang X, Xu Z, Shen X. Development of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2015 May 9.

Abstract

KEY MESSAGE
We reported the first development of Gossypium anomalum -derived microsatellite markers and identification of recombination between sexually incompatible species by a synthesized hexaploid on genome level. To continue to develop improved cotton varieties, it is essential to transfer desired characters from diploid wild cotton species such as Gossypium anomalum to cultivated allotetraploid cotton species. However, interspecific reproductive barriers limit gene transfer between species. In a previous study, we used colchicine treatment to produce a synthesized hexaploid derived from an interspecific hybrid between Gossypium hirsutum and G. anomalum and demonstrated its hybridity and doubled status using morphological, cytological and molecular marker methods. In the current study, to effectively monitor G. anomalum genome components in the G. hirsutum background, we developed 5974 non-redundant G. anomalum-derived SSR primer pairs using RNA-Seq technology, which were combined with a publicly available physical map. Based on this combined map and segregation data from the BC2F1 population, we identified a set of 230 informative G. anomalum-specific SSR markers distributed on the chromosomes, which cover 95.72 % of the cotton genome. After analyzing BC2F1 segregation data, 50 recombination types from 357 recombination events were identified, which cover 81.48 % of the corresponding G. anomalum genome. A total of 203 recombination events occurred on chromosome 11, accounting for 56.86 % of the recombination events on all chromosomes. Recombination hotspots were observed at marker intervals JAAS1148-NAU5100 on chromosome 1 and JAAS0426-NAU998 on chromosome 2. Therefore, all G. anomalum chromosomes are capable of recombining with At chromosomes in G. hirsutum. This study represents an important step towards introgressing desirable traits into cultivated cotton from the wild cotton species G. anomalum.

Features
This publication contains information about 6,654 features:
Feature NameUniquenameType
JAAS3127JAAS3127genetic_marker
JAAS3128JAAS3128genetic_marker
JAAS3129JAAS3129genetic_marker
JAAS3130JAAS3130genetic_marker
JAAS3131JAAS3131genetic_marker
JAAS3132JAAS3132genetic_marker
JAAS3133JAAS3133genetic_marker
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JAAS3135JAAS3135genetic_marker
JAAS3136JAAS3136genetic_marker
JAAS3137JAAS3137genetic_marker
JAAS3138JAAS3138genetic_marker
JAAS3139JAAS3139genetic_marker
JAAS3140JAAS3140genetic_marker
JAAS3141JAAS3141genetic_marker
JAAS3142JAAS3142genetic_marker
JAAS3143JAAS3143genetic_marker
JAAS3144JAAS3144genetic_marker
JAAS3145JAAS3145genetic_marker
JAAS3146JAAS3146genetic_marker
JAAS3147JAAS3147genetic_marker
JAAS3148JAAS3148genetic_marker
JAAS3149JAAS3149genetic_marker
JAAS3150JAAS3150genetic_marker
JAAS3151JAAS3151genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
(86-1 x G. anomalum) x Su8289, BC2F1 (2015)
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1432-2242
eISSN1432-2242
Publication Date2015 May 9
Journal AbbreviationTheor. Appl. Genet.
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article