Development of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes

Publication Overview
TitleDevelopment of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes
AuthorsZhai C, Xu P, Zhang X, Guo Q, Zhang X, Xu Z, Shen X
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Year2015
CitationZhai C, Xu P, Zhang X, Guo Q, Zhang X, Xu Z, Shen X. Development of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2015 May 9.

Abstract

KEY MESSAGE
We reported the first development of Gossypium anomalum -derived microsatellite markers and identification of recombination between sexually incompatible species by a synthesized hexaploid on genome level. To continue to develop improved cotton varieties, it is essential to transfer desired characters from diploid wild cotton species such as Gossypium anomalum to cultivated allotetraploid cotton species. However, interspecific reproductive barriers limit gene transfer between species. In a previous study, we used colchicine treatment to produce a synthesized hexaploid derived from an interspecific hybrid between Gossypium hirsutum and G. anomalum and demonstrated its hybridity and doubled status using morphological, cytological and molecular marker methods. In the current study, to effectively monitor G. anomalum genome components in the G. hirsutum background, we developed 5974 non-redundant G. anomalum-derived SSR primer pairs using RNA-Seq technology, which were combined with a publicly available physical map. Based on this combined map and segregation data from the BC2F1 population, we identified a set of 230 informative G. anomalum-specific SSR markers distributed on the chromosomes, which cover 95.72 % of the cotton genome. After analyzing BC2F1 segregation data, 50 recombination types from 357 recombination events were identified, which cover 81.48 % of the corresponding G. anomalum genome. A total of 203 recombination events occurred on chromosome 11, accounting for 56.86 % of the recombination events on all chromosomes. Recombination hotspots were observed at marker intervals JAAS1148-NAU5100 on chromosome 1 and JAAS0426-NAU998 on chromosome 2. Therefore, all G. anomalum chromosomes are capable of recombining with At chromosomes in G. hirsutum. This study represents an important step towards introgressing desirable traits into cultivated cotton from the wild cotton species G. anomalum.

Features
This publication contains information about 6,654 features:
Feature NameUniquenameType
JAAS3152JAAS3152genetic_marker
JAAS3153JAAS3153genetic_marker
JAAS3154JAAS3154genetic_marker
JAAS3155JAAS3155genetic_marker
JAAS3156JAAS3156genetic_marker
JAAS3157JAAS3157genetic_marker
JAAS3158JAAS3158genetic_marker
JAAS3159JAAS3159genetic_marker
JAAS3160JAAS3160genetic_marker
JAAS3161JAAS3161genetic_marker
JAAS3162JAAS3162genetic_marker
JAAS3163JAAS3163genetic_marker
JAAS3164JAAS3164genetic_marker
JAAS3165JAAS3165genetic_marker
JAAS3166JAAS3166genetic_marker
JAAS3167JAAS3167genetic_marker
JAAS3168JAAS3168genetic_marker
JAAS3169JAAS3169genetic_marker
JAAS3170JAAS3170genetic_marker
JAAS3171JAAS3171genetic_marker
JAAS3172JAAS3172genetic_marker
JAAS3173JAAS3173genetic_marker
JAAS3174JAAS3174genetic_marker
JAAS3175JAAS3175genetic_marker
JAAS3176JAAS3176genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
(86-1 x G. anomalum) x Su8289, BC2F1 (2015)
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1432-2242
eISSN1432-2242
Publication Date2015 May 9
Journal AbbreviationTheor. Appl. Genet.
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article