Molecular mapping of restriction-site associated DNA markers in allotetraploid upland cotton

Publication Overview
TitleMolecular mapping of restriction-site associated DNA markers in allotetraploid upland cotton
AuthorsWang Y, Ning Z, Hu Y, Chen J, Zhao R, Chen H, Ai N, Guo W, Zhang T
TypeJournal Article
Journal NamePloS one
Volume10
Issue4
Year2015
Page(s)e0124781
CitationWang Y, Ning Z, Hu Y, Chen J, Zhao R, Chen H, Ai N, Guo W, Zhang T. Molecular mapping of restriction-site associated DNA markers in allotetraploid upland cotton. PloS one. 2015; 10(4):e0124781.

Abstract

Upland cotton (Gossypium hirsutum L., 2n = 52, AADD) is an allotetraploid, therefore the discovery of single nucleotide polymorphism (SNP) markers is difficult. The recent emergence of genome complexity reduction technologies based on the next-generation sequencing (NGS) platform has greatly expedited SNP discovery in crops with highly repetitive and complex genomes. Here we applied restriction-site associated DNA (RAD) sequencing technology for de novo SNP discovery in allotetraploid cotton. We identified 21,109 SNPs between the two parents and used these for genotyping of 161 recombinant inbred lines (RILs). Finally, a high dense linkage map comprising 4,153 loci over 3500-cM was developed based on the previous result. Using this map quantitative trait locus (QTLs) conferring fiber strength and Verticillium Wilt (VW) resistance were mapped to a more accurate region in comparison to the 1576-cM interval determined using the simple sequence repeat (SSR) genetic map. This suggests that the newly constructed map has more power and resolution than the previous SSR map. It will pave the way for the rapid identification of the marker-assisted selection in cotton breeding and cloning of QTL of interest traits.

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Projects
This publication contains information about 1 projects:
Project NameDescription
P8-RIL-2015
Featuremaps
This publication contains information about 1 maps:
Map Name
Prema x 86-1, RIL (2015)
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DOI10.1371/journal.pone.0124781
eISSN1932-6203
Elocation10.1371/journal.pone.0124781
ISSN1932-6203
Journal AbbreviationPLoS ONE
Journal CountryUnited States
LanguageEnglish
Language Abbreng
Publication Date2015
Publication ModelElectronic-eCollection
Publication TypeJournal Article
URLhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0124781