Working group session:
Structural Genomics
Presentation type:
poster
Authors:
Lin, Zhongxu
Presenter:
Lin, Zhongxu
Correspondent:
Lin, Zhongxu
Abstract:
Molecular genetic map is a prerequisite for understanding the genomic structure and unravelling the qualitative and quantitative genetic control of agronomically important traits in cotton. In order to construction a high-density genetic map, we endeavored to develop kinds of molecular markers to realize this purpose. Besides the markers developed by other international researchers, we developed nearly 5000 EST-SSRs from multi-species including 1129 EST-SSRs recently developed from two 454 sequencing data (G. barbadense acc. 3-79 unnormalized fiber cDNA library and a G. barbadense cv. 7124 normalized root cDNA library). In order to functionally target agronomical traits in cotton, functional markers have been developed from cellulose synthase genes, genes specifically or preferentially expressed during fiber development; IP and IDP markers were developed based on cotton coding sequences; functional markers were also developed for miRNAs and their targets to view their genomic distribution and their expression pattern were characterized during fiber development. With the help of these markers, high-density interspecific genetic map was constructed (1st generation: nearly 1000 loci; 2nd generation: more than 2000 loci; and 3rd generation: more than 5000 loci). The map has been effectively applied in genomic structure explanation, general QTL mapping, QTL fine mapping, and introgression lines development by MAS.