Working group session:
Functional Genomics
Presentation type:
poster
Authors:
Hao, Juan; Sun, Yuqiang
Presenter:
Hao, Juan
Correspondent:
Sun, Yuqiang
Abstract:
Cotton fibers supply the main raw material to textile industry, fiber initiation and elongation are the key determinants of fiber output and quality in cotton. Although transcriptome and functional genomics have made great progress, the mechanism of fiber development is still not well understood. With Gossypium arboreum, G. stocksii and their somatic hybrid as our research materials, the cDNA-AFLP was used to analyze their fiber development transcriptome. At present, a total of 598 differentially expressed genes were isolated. This project intends to screen and clone the genes involved in fiber development by bioinformatic and expression analyses firstly. Then construct the expression vector VIGS and transform into G. hirsutum. Functional candidate genes will be validated by analyzing transgenic cotton fiber development and endogenous gene expression. For further analysis of gene function and regulatory mechanism, CRISPR/Cas system and overexpression strategies will be applied in cotton and Arabidopsis. The fibers, root hairs and trichomes of transgenic plants will be analyzed in detail. Ultimately, we hope to explore the key genes for cotton fiber quality improvement, and elucidate the regulatory mechanism of fiber development preliminarily.