Analysis of biochemicals and transcriptome associated with brown and white fibre development in Gossypium hirsutum L.

Working group session: 
Functional Genomics
Presentation type: 
poster
Authors: 
Gong, Wenfang; Du, Xiongming; Ji, Rong; Sun, Junling; He, Shoupu; Pang, Baoyin
Presenter: 
Gong, Wenfang
Correspondent: 
Du, Xiongming
Abstract: 
Natural colored cotton is an important cotton germplasm and potential dye-free textile material. However, the study involved the molecular mechanism of fiber development and pigmentation formation are very less. RT-white and Brown1-61, the near-isogenic lines of upland cotton with white and brown fibres, were analyzed. We found that immature fibres of white and brown cotton at 28 days post-anthesis (dpa) contained flavones, whereas brown cotton fibres at 13dpa, 17dpa, 23dpa, leaf, flower, and bud contained flavonols. But Brown1-61 mature fibres may contain procyanidin (tannin).Using the RNA-seq technology which provides a powerful tool to reveal the whole transcriptome profiling of developmental colored fiber, the most important pigment biosynthesis pathways in plant, including "flavonoid biosynthesis" and "anthocyanins biosynthesis" pathways were analyzed. The result demonstrated that the pigmentation formation in brown color cotton fiber was possibly the consequence of interaction of oxidized tannins and glycosylated anthocyanins.