Working group session:
Germplasm and Genetic Stocks
Presentation type:
poster
Authors:
Yang, Xinlei ; Zhou, Xiaodong ; Wang, Yunpeng ; Wang, Xingfen ; Li, Zhikun ; Wu, Liqiang ; Zhang, Yan ; Liu, Hengwei ; Zhang, Guiyin ; Ma, Zhiying
Presenter:
Yang, Xinlei
Correspondent:
Ma, Zhiying
Abstract:
A BC1 population containing 115 individuals from a cross between Gossypium hirsutum cv. CCRI8 and G. barbadense cv. Pima 90-53 was established and 519 SSR markers, two conserved intron scanning primers (CISP) and transcript-derived fragments (TDF) amplified from 156 Apo I/Taq I selective primer combinations were used to construct a genetic linkage map. The map consisted of 579 markers distributed on 56 linkage groups. Accounting for 83.4% of the cotton genome, it covered 4,168.72 cM, with an average distance of 7.19 cM between markers. Based on this newly constructed map of tetraploid cotton, we performed quantitative trait loci (QTL) mapping of fiber quality traits from the BC1 and its derived BC1F2 lines. A total of 46 fiber quality QTL were detected on 17 chromosomes, explaining 7.72% to 23.73% of the phenotypic variation. The Pima 90-53 offered 15 QTL alleles with positive additive effects and four with negative additive effects for fiber quality traits. Of these, the qFS9-1 and qFS9-2 explaining 15.71% and 14.42% of the phenotypic variation were detected in the common interval from NAU1092a to NAU6101b in BC1 population and its derived BC1F2 lines, grown at Baoding suburb in 2007 and Xinji suburb in 2008. In order to better utilize fiber strength of Pima 90-53, the stable QTL of fiber strength was validated by multi-populations, multi-generations, multi-sites and multi-years. Three populations (five generations) were employed to validate QTL for fiber strength in cotton grown at 4 years representing six different environments. The first one comprising 131 BC1F2 population developed by backcrossing with CCRI 8 and two its derived family lines was grown at Baoding suburb in 2010, Qingxian county and Guangzong county in 2011, Qingxian county and Nangong county in 2012. The second and third, including 176 BC1 population and 350 F2 population derived from a cross G. hirsutum cv. Han 208 and Pima 90-53 were grown at Sanya suburb in 2013. Fortunately, this QTL was further dissected into two QTL at this region in BC1F2 population. The qFS9-3 and qFS9-4 explaining 16.27% and 17.85% of the phenotypic variation were detected in the common interval from NAU 2395 to NAU1092a in BC1F2 and its derived BC1F2:3 lines at two environments, including Baoding suburb in 2010 and Qingxian county in 2011, respectively. Also, The qFS9-5 and qFS9-6 explaining 12.13% and 14.75% of the phenotypic variation were detected in the common range from NAU 2395 to NAU1092 in BC1 and F2 population at Sanya in 2013. All QTL showed positive additive effects except the qFS9-5. So, this is a very stable and common QTL with the same interval markers, and we proposed qFSA9 as the name for these QTL. A CI of qFSA9 was 1.37-5.92 cM with two SSR markers (NAU2395 and NAU1092) and the largest LOD value was 5.00 for this QTL in BC1 grown at Baoding suburb in 2007. The SSR markers linked to the QTL can be utilized to marker-assisted selection of fiber strength.