Working group session:
Structural Genomics
Presentation type:
oral
Authors:
Shoupu, He; Gaofei, Sun; Zhaoe, Pan; Junling, Sun; Yinhua, Jia; Wenfang, Gong; Liru, Wang; Baoyin, Pang; Xiongming, Du
Presenter:
Shoupu, He; Xiongming, Du
Correspondent:
Xiongming, Du
Abstract:
Simple sequence repeats (SSRs) have been applied as the most popular molecular markers for cotton for many years; however, few markers or quantitative trait locus (QTLs) have been utilized in the breeding process. The draft sequences of the A and D diploid cotton genomes, known as donor genomes of tetraploid cotton, provide a great opportunity to better understand the distributional and structural profile of microsatellites in the two genomes. Global assessment of all microsatellites and homology analysis in the two genomes revealed the complex genomic structure of Gossypium. We further used a critical strategy to screen and physically map all the published SSR markers in the genomes, where two multipurpose creditable marker maps were constructed. A comparison between MISA-mined microsatellites and mapped published markers suggested that microsatellites still have high potential value for the cotton genome. A preliminary meta-QTL relocation based on our map provided accurate position information for further fine mapping and gene cloning. This study reports for the first time the complete distribution and homology of microsatellites in two diploid cotton genomes based on the genome sequence, which provides new insight and better understanding of the use of microsatellites in evolutionary and genetic studies.