Working group session:
Germplasm and Genetic Stocks
Presentation type:
poster
Authors:
Javaid, Areej; Awan, Faisal S.; Azhar, Faqir M.; Khan, Iftikhar A.
Presenter:
Javaid, Areej
Correspondent:
Javaid, Areej; Awan, Faisal S.
Abstract:
Drought in conjunction with high temperature is an important environmental constraint to cotton production. Development of cotton varieties with increased fitness to adverse environmental conditions has been proposed as economical and durable strategy for getting reliable yields. Molecular marker technology holds great promise for crop improvement exercise particularly in cotton where repeated cycles of directional selection for high yield and earliness might have caused loss of genetic diversity. In the present study, 11 SSR primers were used to estimate genetic divergence for water stress tolerance among 22 cotton genotypes. These genotypes were previously selected on the basis of drought induced morpho-physiological responses. Two SSR primers (JESPR-247 and JESPR-291) out of 11 were able to discriminate cotton varieties thus revealing informative primers to be 18%. Total detected loci were 33 with an average of 3 loci per primer. The number of amplified fragments detected at each locus ranged from one (JESPR-284) to six (JESSPR-302). Allelic diversity found in the experimental material was low i.e. 0.1. In this study, genetic similarity coefficients ranged from 0.87 to 1.00 where 17 varieties possessed similarity coefficient 1.00, thus showing that major portion of the genome was similar due to related genetic background among genotypes. The cluster analysis arranged 21 genotypes in two main groups whilst CIM-109 remained ungrouped being divergent from remaining varieties. Clustering of 17 genotypes in one major cluster confirmed similarity coefficients to be 1.00. Overall,low genetic diversity was found in 22 cotton genotypes. It had been suggested to apply more polymorphic SSR markers to explore the workable genetic variation among the screened cotton genotypes.