Working group session:
Germplasm and Genetic Stocks
Presentation type:
oral
Authors:
Kushanov, Fakhriddin; Makamov, Abdusalom; Darmanov, Mukhtor; Turaev, Ozod; Tulanov, Akmal; Shermatov, Shukhrat; Buriev, Zabardast; Abdukarimov, Abdusattor; Abdurakhmonov, Ibrokhim
Presenter:
Correspondent:
Kushanov, Fakhriddin; Abdurakhmonov, Ibrokhim
Abstract:
Molecular marker technology offers improved selection strategies in plant breeding. In the last decade, the DNA marker technology has been applied for identification QTLs associated with fiber quality and donor lines (with superior quality) for the purpose of developing new cotton lines in cotton breeding. The application of DNA marker technology to cotton for germplasm studies has identified novel quantitative trait loci (QTL) associated with fiber quality and unique donors with superior quality. Twenty six germplasm accessions, as donor lines and more than 10 varieties, as the recipient parents were selected for mobilization of novel QTL alleles from unique donors to the genetic background of commercial Uzbek cotton cultivars through marker assisted backcrossing (MAB). In each backcross generations, allele mobilization to hybrids was controlled via molecular markers, respectively and hybrids with marker bands were selected. Selected hybrid fibers were analyzed for their quality, including length (FL), fiber strength (FS), micronaire (MC), fiber elongation (FE), fiber uniformity (FU) using High Volume Instrument (HVI). The results of HVI analyses were indicated good fiber qualities of selected hybrids. In 2013, between two combinations of F1BC5 generations, two lines named “Ravnaq-1” and “Ravnaq-2” (translated as “Advance”) were selected and sent to the State Variety Testing Committee of Uzbekistan for larger filed trails in different environments. Both varieties possess higher fiber strength and improved length. For instance, “Ravnaq-1” has superior fiber quality with improved fiber strength (37 g/tex) and staple length (38 mm) compared to its recurrent parent “Andijan-35” which has 32 g/tex fiber strength and 35 mm staple length. Similarly, in “Ravnaq-2” molecular markers effectively mobilized superior fiber quality loci that improved fiber strength by 17% and staple length by 9% compared to its recurrent parent “Mekhnat”. In conclusion, our markers and donors have been proved to be useful in MAS to obtain superior cotton cultivars within short times, which bear novel untaped fiber quality loci.