Working group session:
Germplasm and Genetic Stocks
Presentation type:
poster
Authors:
Shi, Yuzhen; Li, Wentan ; Yuan, Youlu
Presenter:
Shi, Yuzhen; Li, Wentan
Correspondent:
Yuan, Youlu
Abstract:
Genetic linkage maps play fundamental roles in understanding genome structure, explaining genome formation events during evolution, and discovering the genetic bases of important traits. A new high density allotetrploid cotton genetic map of simple sequence repeat (SSR) markers was developed by using BC1F1 population CCRI36×(CCRI36×Hai1) including 135 individuals. The genetic map comprised 2292 loci and covered 5115.16 centiMorgan (cM) of the cotton genome (AD) with an average marker interval of 2.23 cM. Of the 2292 loci, 1025 (44.72%) were mapped to 13 At-subgenome chromosomes, covering 2420.71 cM (47.32%), and 1268 (55.28%) mapped to 13 Dt-subgenome chromosomes, covering 2694.45 cM (52.68%). 62.92 % duplicated loci sufficiently bridged 13 expected homologous At/Dt chromosomes. 53.14% marker loci were newly discovered in this map compared with the published 5 high-density cotton genetic maps constructed mainly by SSR markers. By comparison with the whole-genome marker map based on the D genome sequence, the overall marker order for 1465 common loci on this new map agree well with the marker order on D genome, indicating high level of homology between the diploid D genome and the tetraploid cotton genetic map. This new independent high density SSR genetic map will be facilities, as a valuable genomic resource, for research on structural and functional genomics of allotetraploid cotton.