Working group session:
Functional Genomics
Presentation type:
poster
Authors:
Ayubov, Mirzakamol; Buriev, Zabardast; Ubaydullaeva, Khurshida; Makamov, Abdusalom ; Shapulatov, Umidjon ; Soliev, A’zamjon ; Norov, Tokhir ; Darmanov, Mukhtor; Mirzaakhmedov, Muhammad ; Yashinov, Ansor ; Abdukarimov, Abdusattor ; Pepper, Alan ; Abdurakhmonov, Ibrokhim
Presenter:
Correspondent:
Ayubov, Mirzakamol; Pepper, Alan ; Abdurakhmonov, Ibrokhim
Abstract:
Phytochromes are family of photoreceptors involved in regulation of complex developmental and molecular processes in plant cells. Recently, we characterized phytochrome gene family in cotton genome including PHYA, PHYB, PHYC and PHYE genes. Each phytochrome is responsible for many processes in cotton which directly related to light effects such as vegetative growth, plant architecture, photoperiodic flowering, maturity, fiber quality, tolerance to environmental stresses and productivity. It is known that the red light photoreceptor PHYB gene is responsible for inhibition of plant flowering. In order to study exact biological function of cotton PHYB gene(s), we designed synthetic short oligonucleotide duplex for cotton PHYB gene that introduced to cells, should specifically suppress the targeted gene expression. A binary pART27 vector construct bearing synthetic phyB cassette (SynB, driven by 35S promoter) and kanamycin resistance gene marker was developed and somatically transformed into Coker 312. RNAi plants were obtained using somatic embryogenesis. Candidate RNAi plants bearing the synthetic PHYB RNAi duplex were verified using PCR reaction that amplifies specifically phyB duplex insertions from the vector construct. Phenotypic observations of SynB RNAi T1-5 plants showed early flowering and fiber Micronaire (MIC) improvement compared to wild-type and null segregant controls. Our efforts helped to accurately regulate gene function and related agronomically important traits in complex plant genomes such as allopolyploid cotton. Details of phenotypic and molecular characterization of cotton PHYB gene RNAi plants will be discussed.