Working group session:
Functional Genomics
Presentation type:
poster
Authors:
Chi, Ji-na; Zhen, Jun-bo; Cai, Xiao; Zhang, Jian-hong; Liu, Cun-jing; Tian, Hai-yan; Cui, Rui-min; Tang, Li-yuan; Liu, Lin-lin; Zhang, Xiang-yun
Presenter:
Chi, Ji-na
Correspondent:
Zhang, Xiang-yun
Abstract:
Cotton （Gossypium spp.）is the world’s most important fiber crop plant. Cotton fiber has been used in the textile and many other industies diffusely. Cotton fiber qualities including length, strength and fineness are known to be controlled by genes affecting cell elongation and secondary cell wall (SCW) biosynthesis. MicroRNAs are a class of small non-coding RNAs that down-regulate gene expression in a sequence specific manner to control plant growth and development. The identification and characterization of microRNAs are critical steps in finding their target genes and elucidating their functions. Here, we elucidated microRNAs from two developmental time points during elite upland cotton Ji228 fiber development by high-throughput sequencing. 41 conserved microRNAs belonging to 19 microRNAs families and 148 novel microRNAs were discovered, and 725 potential targets genes were predicted. On the other hand, 43,809 unigenes and 3,463 novel genes were mined from 7 development time points during elite upland cotton Ji228 fiber development by RNA-seq and DEG. 3 categories and 28 classes were found according to the expression profile of sequencing and gene expression of dynamic change in fiber development. MicroRNA-mRNA interactions potentially relevant for fiber development were explored using anti-correlation expression analysis and microRNAs target prediction algorithms. 9 microRNAs and their target genes were get from the relationship between the differences of microRNAs and differences mRNA. The data suggest critical roles of transcription relation and microRNA-mediated pathways in fiber development.