Working group session:
Functional Genomics
Presentation type:
poster
Authors:
Xie, Zongming; Xie, zongming; Li, Yue; Chen, Shouyi; Chen, Shouyi
Presenter:
Xie, Zongming; Xie, zongming
Correspondent:
Xie, Zongming; Xie, zongming; Chen, Shouyi; Chen, Shouyi
Abstract:
Trihelix factors constitute a family of plant-specific transcription factors, play important roles in light-regulated responses and other developmental process and in responses to environmental stresses. In this study, a novel trihelix gene, named GhSIP1, was isolated from Gossypium hirsutum, and further characterized its roles in abiotic stress tolerance. GhSIP1 can be induced by various abiotic stresses, and the encoded proteins were localized in nuclear region. In Arabidopsis protoplast assay, no transcriptional activation activity detected for GhSIP1. DNA binding analysis using Electrophoretic Mobility Shift Assay（EMSA）showed that GhSIP1 could bind to Box, Site1, GT-1box, GT2-Box, GT3-Box, GT-3b, MBS1 and MRE4. Overexpression of the GhSIP1 improved plant tolerance to salt stress in transgenic Arabidopsis plants. Moreover, GhSIP1-transgenic plant seedlings had longer root length compared to Col-0 under ABA treatment. Many stress-responsive genes were altered in GhSIP1-transgenic plants. The results indicate that GhSIP1 confer high salt tolerance through regulation of a common set of genes and specific sets of genes.
Key words: GhSIP1;tihelix factor;salt tolerance;Gossypium hirsutum;transgenic Arabidopsis