Working group session:
Functional Genomics
Presentation type:
poster
Authors:
ge, xiaoyang; wu, jiahe; li, fuguang
Presenter:
ge, xiaoyang
Correspondent:
wu, jiahe; li, fuguang
Abstract:
In process of cotton (Gossypium hirsutum L.) somatic embryo development (SED), quite few embryos are able to normally mature and germinate, and successfully regenerate plantlets. Many efforts have been implemented for increasing regeneration rate, however, the maturation rate of somatic embryo is still very low. Here, a global analysis of proteome dynamics between globular and cotyledonary embryos was performed using isobaric tag for relative and absolute quantification (iTRAQ) method to better understand mechanisms underlying somatic embryo development for increasing cotton regeneration rate. A total of 5718 proteins were identified by mass spectrometry (MALDI-TOF/TOF) analysis , 209 proteins of which exhibited differential expression, including 102 upregulating proteins and 107 downregulating proteins. Based on Gene Ontology annotation, the indentified proteins could be classified into 22 biological processes and respectively localized to chloroplast, cytoplasm, thylakoid and so forth. Most of these proteins were implicated in cellular component organization or biogenesis, cellular processes and metabolic processes. Proteomic analysis of SED indentified several important proteins major involving in signal transduction, energy production and conversion, and carbohydrate metabolism. The expression level of the corresponding genes was further characterized by employing quantitative Real-time PCR. Interestedly,Pathway analysis demonstrated that abscisic acid（ABA）, giberellic acid (GA), jasmonic acid（JA） are involved in various physiological activities and has a distinct influence on SED. When exogenous hormones added in medium, the results showed that ABA promoted somatic embryo maturation, GA was negative to SED, and JA accelerated SED. The protome dynamics analysis provides a better understanding of the possible regulation mechanisms of plant hormone and some important protein function in cotton SED.