The identification of the cotton monosomics of Uzbek cytogenetic collection using SSR markers

Germplasm and Genetic Stocks

poster

Sanamyan, Marina; Ernazarova, Dilrabo; Makamov, Abdusalom; Usmonov, Dilshod; Saha, Sukumar; Stelly, David; Abdurakhmonov, Ibrokhim

Sanamyan, Marina

Sanamyan, Marina; Ernazarova, Dilrabo; Makamov, Abdusalom; Usmonov, Dilshod; Saha, Sukumar; Abdurakhmonov, Ibrokhim

The identification of the cotton monosomics of Uzbek cytogenetic collection using SSR markers Marina Sanamyan1,3, Dilrabo Ernazarova2, Abdusalom Makamov3, Dilshod Usmonov3, Sukumar Saha4, David Stelly5, Ibrokhim Abdurakhmonov3. 1National University of Uzbekistan, Tashkent, Uzbekistan. 2Institute of Genetics and Plant Experimental Biology, Academy of Sciences, Tashkent, Uzbekistan. 3Center of Genomics and Bioinformatics, Academy of Sciences of Uzbekistan, Ministry of Agriculture and Water resources, and “UzCottonIndustry” Association, Tashkent, Uzbekistan. 4USDA-ARS, Crop Science Research Laboratory, Mississippi State, MS, USA. 5Texas A&M University, College Station, TX 77843 USA In Uzbekistan, Cytogenetic collection National University (CCNUz), a total of 94 primary monosomics of G. hirsutum L. from the common genetic background of the highly inbred line L-458 were developed through irradiation of cotton seeds by thermal neutrons or pollen gamma-irradiation in M1, M2 and M3 generations. Some of these monosomics were characterized for morphological traits and cytogenetic characteristics. Further, several monosomics were identified using a well-defined tester set of translocation lines of Cytogenetic collection of the USA. Considering a lot of DNA markers have already been assigned to the individual chromosomes of G. hirsutum L., we aimed to utilize chromosome specific simple sequence repeat (SSR) markers to identify and reconfirm the chromosome specificity of all cytogenetic resources in CCNUz collection. Toward this goal, we crossed our monosomics lines with doubled haploid Pima 3-79 line (G. barbadense) and isolated F1 hybrid plants that are monosomic for substituted Pima chromosomes using cytogenetic analysis. Assignment of SSR markers was straightforward and in a manner described in previous reports that utilized a PCR amplification of chromosome specific markers in the genomic DNAs of hybrid plants. We used chromosome specific SSR markers to identity BC0F1 aneuploid plants from CCNUz collection. Results suggested that we identified monosomic lines associated with chromosome 2 (Mo11, Mo16 and Mo19), chromosome 4 (Mo70, Mo71, Mo76, Mo81, Mo89 and Mo90), and chromosome 6 (Mo13 and Mo67). Molecular identification of remaining cytogenetic stocks is in progress and will be discussed.