Working group session:
Structural Genomics
Presentation type:
oral
Authors:
Xu, Peng; Shen, Xinlian; Ni, Wanchao
Presenter:
Xu, Peng
Correspondent:
Shen, Xinlian
Abstract:
Abstract
Gossypium klotzschianum carries elite alleles and is a D-genome diploid species of originating from the Galapagos Island. This species harbors lethal genes that cause inconsistency in apical bud growth. In this study, we overcame the obstacle and established a BC1F2 population derived from (Simian 2×G. klotzschianum)×Simian 2 (G. hirsutum). The simple sequence repeat (SSR) markers BNL2652, NAU1201 and NAU1141 exhibited significant correlation with fiber length and micronaire value. The BC2F3 and BC2F4 populations were then developed by crossing BC1F2 individuals containing target segments of chromosome 13 from G. klotzschianum and the recurrent parent Simian 2. Quantitative trait loci (QTL) associated with fiber quality properties in two generations were identified using the Cartographer (V2.5) software and composite interval mapping. A total of five QTL were detected in both generations. The fiber length QTL identified in BC1F2 population, qFL-13-1, was confirmed in BC2F3 and BC2F4, which accounted for phenotypic variance of 7.3% and 7.0%, respectively. The G. klotzschianum allele increased fiber length. The QTL for fiber micronaire detected in BC1F2 population, qFMIC-13-1, was also confirmed in BC2F3 and BC2F4, which accounted for phenotypic variance of 11.2% and 7.2%, respectively. The G. klotzschianum allele decreased the fiber micronaire value. Three other QTLs for fiber quality properties were also detected in both generations. Furthermore, a pair of putative homologous QTL for fiber length, qFL-13-1 and qFL-18-2, was identified. This study provides valuable resources for effective utilization of potential elite genes from G. klotzschianum.