Working group session:
Functional Genomics
Presentation type:
poster
Authors:
Shen, Xinlian; Feng, Juan ; Zhang, Xianggui
Presenter:
Shen, Xinlian
Correspondent:
Abstract:
Abstract: CIPK ( calcineurin B-like calcium sensor interacting the protein kinase) is a group of typical serine / threonine protein kinase targeting calcium sensor calcineurin B-like protein-specific. In the previous study, we obtained the transcriptome of Gossypium.aridum associated with salt stress. It was founded that a CIPKrelated gene had high level of expression abundance. .Then, a full-length cDNA encoding a CIPKs homologue (GarCIPK) was isolated from G.aridum by in-silico cloning and RT-PCR techmology. The deduced GarCIPK protein had 449 amino acids with an estimated molecular mass of 51.12kD and an isoelectric point of 8.13. The putative kinase N-terminal catalytic domain of GarCIPK contained a serine / threonine protein kinase domain. The conserved 24-amino acid motif within C-terminal non-kinase regions was also found in GarCIPK. In order to comfirm its putative function, the GarCIPK gene which was driven by CaMV35S promoter and stress-specific promoter RD29A were transferred into tobacco separately by agrobacterium-mediated transgenic technology. The trangeneic plants of T1 generation were used to identify salt and drought tolerance. The results showed that transgenic plants had enhanced tolerance to salt and drought stress.