Working group session:
Structural Genomics
Presentation type:
poster
Authors:
kuraparthy, vasu; Andres, Ryan
Presenter:
Andres, Ryan
Correspondent:
kuraparthy, vasu
Abstract:
Cytogenetic discoveries in cotton are very slow because of lack of efficient chromosome spreading techniques, low mitotic index and unavailability of single copy probes. Besides cotton chromosomes are numerous and small which result in poor mitotic spreads. Current spreading techniques are laborious, time consuming and require skilled labor. A simple and new chromosome spreading technique was developed involving gas chamber and enzymatic treatments. The new technique is highly efficient, simple and does not need skillful hands and is compatible for FISH and GISH in cotton.