Working group session:
Functional Genomics
Presentation type:
oral
Authors:
Kim, Hee Jin; Triplett, Barbara; Zhang, Hong-Bin; Lee, Mi-Kyung; Hinchliffe, Doug; Li, Ping; Fang, David
Presenter:
Kim, Hee Jin
Correspondent:
Kim, Hee Jin
Abstract:
Cotton fibers are unicellular seed trichomes and consist of almost pure cellulose. During the transition from elongation to secondary cell wall (SCW) biosynthesis stage in developing cotton fibers, cellulose biosynthesis is significantly increased. Cellulose synthase catalytic subunits (CesAs) play an important role for cellulose biosynthesis. Although the first two cellulose synthase catalytic subunits (CesAs) were isolated from developing cotton fibers, it is not clear how many CesAs are involved in SCW biosynthesis in fibers, or how these genes are regulated during cell wall biogenesis. Based on diploid Arabidopsis genome data that a trio of CesAs is required for SCW biosynthesis, a minimum of three pairs of homeologous CesA genes are expected to be expressed during SCW biosynthesis in allotetraploid G. hirsutum. To date, two pairs of homeologous SCW CesA genes (GhCesA1-Dt, GhCesA4 or GhCesA1-At, GhCesA2-Dt, and GhCesA2-At) have been characterized from G. hirsutum fibers. We have characterized a pair of homeologous GhCesA2 genes and analyzed promoter activity of GhCesA4. Temporal and spatial expressions, transcriptional regulation and potential alternative splice of SCW GhCesAs will be presented.
Kim et al. (2012) Cloning and characterization of homeologous cellulose synthase catalytic subunit 2 genes from allotetraploid cotton (Gossypium hirsutum L.). Gene, 494: 181-189.
Kim et al. (2011) Functional analysis of Gossypium hirsutum cellulose synthase catalytic subunit 4 promoter in transgenic Arabidopsis and cotton tissues. Plant Science, 180: 323-332.