Working group session:
Comparative Genomics and Bioinformatics
Presentation type:
oral
Authors:
Verma, Surender ; Logan-Young, Carla; Pepper, Alan; McCormick, Ryan; Verma, Surender ; Logan-Young, Carla; Pepper, Alan; McCormick, Ryan; Percy, Richard ; Yu, John; Percy, Richard ; Yu, John
Presenter:
Verma, Surender ; Verma, Surender
Correspondent:
Pepper, Alan; Pepper, Alan; Yu, John; Yu, John
Abstract:
Recent developments in next-generation sequencing (NGS) technology have lowered the cost of sequencing per base and enabled whole genome re-sequencing, genome-wide association studies, and for some species, unprecedented discovery of molecular markers. For species with large, complex genomes, genotyping-by-sequencing (GBS) of reduced representation libraries has emerged as a promising tool for association studies and genomics-assisted breeding. We used a simplified GBS approach to genotype recombinant inbred lines (RILs) of an interspecific cross between Gossypium hirsutum x G. barbadense (TM-1 x 3-79) to discover novel SNPs and develop an interspecific linkage map. The GBS approach presented here provides a powerful tool for developing informative markers in species without a sequenced genome, while also providing a valuable resource for anchoring physical maps and ordering scaffolds from whole genome sequencing projects. The GBS approach will have broad applications in SNP marker-assisted breeding in taxa with limited prior knowledge of the genome or genetic diversity.