Working group session:
Functional Genomics
Presentation type:
poster
Authors:
Liu, Chuanxiang ; Lin, Zhongxu ; Zhang, Xianlong
Presenter:
Liu, Chuanxiang
Correspondent:
Lin, Zhongxu
Abstract:
QTL mapping is an important part of marker-assisted selection breeding. There are many studies on the QTL related to cotton fiber yield and quality, but most of them based on a DNA level, which may appear some null QTLs. However, QTL mapping based on transcriptome map at cDNA level can be more reliable. An interspecific transcriptome map of allotetraploid cotton was developed based on an F2 population [Emian22 ×3-79] by amplifying cDNA using EST-SSRs. The map was constructed by using 5DPA developing fiber. This map contained 242 markers which distributed in 32 linkage groups (26 chromosomes). The full length of this map was 1938.72 cM with a mean marker distance of 12.47 cM. Functions of some ESTs have been annotated through searching homologous sequences. Some of the makers related to the differentiation and elongation of cotton fiber, most of them related to basic metabolism. This study proved that constructing transcriptome map by amplifying cDNA using EST-SSRs is a very simple method, and the transcriptome map will be a power tool for mapping eQTLs of fiber quality.