Working group session:
Breeding and Applied Genomics
Presentation type:
oral
Authors:
Guo, Wangzhen; Zhang, Tianzhen
Presenter:
Guo, Wangzhen
Correspondent:
Zhang, Tianzhen
Abstract:
Sea-island cotton (Gossypium barbadense L.) has superior fibre quality properties such as length, fineness and strength, while Upland cotton (Gossypium hirsutum L.) is characterized by high yield. To reveal features of Upland cotton and Sea-island cotton fibre cells, 418 differential genes expression profiles during fibre cell elongation and in secondary wall deposits were established using cDNA microarray technology. Comparative analysis of transcriptomes by cDNA microarray showed 322 genes were differentially expressed at the fibre cell elongation stage. Of these, 198 genes were higher in TM-1 and 116 genes were higher in Hai7124, respectively. GO and KEGG analysis of the differentially expressed genes indicated biological progress primarily involved in carbohydrate metabolism, lipid metabolism and the response to phytohormone stimulus. Additionally, sucrose and starch metabolism, flavonoid biosynthesis, phenylalanine metabolism, ascorbate and aldarate metabolism, pyrimidine metabolism and the phosphatidylinositol signalling system were involved in the fiber elongation stage. and a total of 32 differentially expressed genes were mapped to regions previously reported to contain quantitative trait loci affecting fibre qualities. This research provides a valuable genomic resource to deepen our understanding of the molecular mechanisms of cotton fibre development, and may ultimately lead to improvements in cotton fibre quality and yield.