Gossypium hirsutum (AD1) 'CSX8308' genome HGS_v1.1
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Overview
About the assembly Main assembly consisted of 114.46x of PACBIO coverage (15,367 bp average read size), and was assembled using MECAT and the resulting sequence was polished using ARROW. A total of 108,262 unique, non-repetitive, non-overlapping 1 KB syntenic markers were generated using the G. hirsutum v2.0 genome (Chen et al., 2020) release and aligned to the polished CSX8308 assembly. Contig breaks were identified as an abrupt change in linkage group. A total of 14 breaks were made. The broken contigs were then ordered, oriented, and assembled into 26 chromosomes using the version 2.0 G. hirsutum syntenic markers. A total of 296 joins were made during this process. The HiC library was aligned to the integrated chromosomes, and several minor rearrangements were made. Adjacent alternative haplotypes were identified on the joined contig set. AltHap regions were collapsed using the longest common substring between the two haplotypes. A total of 112 adjacent altHaps were collapsed. Care was taken to ensure that telomere was properly oriented in the chromosomes, and the resulting sequence was screened for retained vector and/or contaminants. Finally, Homozygous SNPs and INDELs were corrected in the release sequence using ~65x of Illumina reads (2x150, 400bp insert). Genome Information
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The chromosomes (pseudomolecules) for G. hrisutum CSX8308 genome. These files belong to the HGS (Phytozome) Assembly v1.0
Functional Analysis
Functional annotation files for the Gossypium hirsutum CSX8308 Genome v1.0 are available for download below. The Gossypium hirsutum CSX8308 Genome v1.0 proteins were analyzed using InterProScan in order to assign InterPro domains and Gene Ontology (GO) terms. Pathways analysis was performed using the KEGG Automatic Annotation Server (KAAS). Downloads
Genes
The predicted gene model, their alignments, and proteins for G. hirsutum 'CSX8308' genome. These files belong to the HGS (Phytozome) Assembly v1.0
Homology
Homology of the Gossypium hirsutum CSX8308 genome v1.1 proteins was determined by pairwise sequence comparison using the blastp algorithm against various protein databases. An expectation value cutoff less than 1e-6 for the Arabidoposis proteins (Araport11, 2022-09), UniProtKB/SwissProt (Release 2024-03), and UniProtKB/TrEMBL (Release 2024-03) databases. The best hit reports are available for download in Excel format. Protein Homologs
Markers
Marker alignments were performed by the CottonGen Team of Main Bioinformatics Lab at WSU. The alignment tool 'BLAT' was used to map marker sequences from CottonGen to the Gossypium hirsutum CSX8308 v1.1 assembly. Markers required 90% identity over 97% of their length. For SSRs & RFLPs, gap size was restricted to 1000bp or less with less than 2 gaps. For dbSNPs and Indels gap size was restricted to 2bp with less than 2 gaps. The available files are in GFF3 format. Markers available in CottonGen are linked to JBrowse.
Transcript Alignments
Transcript alignments were performed by the CottonGen Team of Main Bioinformatics Lab at WSU. The alignment tool 'BLAT' was used to map transcripts to the G. hirsutum CSX8308 v1.1 genome assembly. Alignments with an alignment length of 97% and 90% identify were preserved. The available files are in GFF3 format.
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